Mesenchymal Stem Cells Alleviate Moderate-to-Severe Psoriasis by Reducing the Production of Type I Interferon (IFN-I) by Plasmacytoid Dendritic Cells (pDCs)
MSC infusion affected neutrophil function and reduced the production of IFN-α by pDCs. (a) The changes of neutrophil in mouse blood (a), spleen (b), and dLN (c) were identified as CD11b+Ly6G+ after MSC infusion. (a) Relative gene expression of proinflammatory cytokine (IL-17) and antimicrobial peptide (CRAMP and MPO) in splenic isolated neutrophils from all groups. The IL-17 production of splenic isolated neutrophil supernatants was evaluated by ELISA. (e) WT splenic pDCs were incubated with cell-free supernatants from resting control, MSC-treated, and untreated mouse splenic neutrophils, respectively; IFN-α transcripts (left) and protein (right) were assessed by q-PCR and ELISA. Data were pooled from 3 independent experiments. Data was represented as .# and ## (control group vs. the untreated group in all cases); and (untreated group vs. the MSC-treated group).