MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing
MSC-based therapy is macrophage-dependent and enhances cutaneous wound healing. (a) Representative light field photographs of cutaneous wounds in normal mice or macrophage-depleted mice after treatment with PBS, BMMSCs or JMMSCs (a1); the whole cutaneous wound is outlined in a dashed line. Percentage of the wound closure on day 3 to day 12 in reference to the day 0 wounds from the groups described in the left figures (a2) (). (b) Representative H&E image from a cutaneous wound at day 12, the green arrows indicating the wound edge. (c) Masson trichrome (c1) showing collagen deposition at day 12 and quantification of collagen index (c2) (). (d) Immunostaining of CD31 and proliferating cell nuclear antigen (PCNA) at day 12 of skin wound (). (e) Quantification of immunostaining of CD31 and PCNA positively stained area percentages at day 12 of skin wound (). Scale bars: 500 μm (b) and 50 μm (c, d). , , and . Error bars are .