MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing

Figure 3

Uptake of MSC-secreted exosomes by macrophages promotes M2 polarization. (a) The expression of CD63 and CD81 in BMMSC-ex and BMMSCs (a1) and JMMSC-ex and JMMSCs (a2) assessed by western blot analysis. (b) Exosomes (PKH26, red) from BMMSCs or JBMMSCs entered into macrophages (CD68, green). (c) CD14 (green) and CD163 (red) staining of macrophages after being cocultured with BMMSC-, BMMSC/siRab27a-, or BMMSC-derived exosomes. Macrophages cocultured with BM/siRab27a showed less CD14 and CD163 double-positive cells compared to the BMMSC or BMMSC-ex group. (d) CD206-positive macrophages after being cocultured with exosomes and BMMSCs were assessed by flow cytometric analysis (). CD206 expression increased compared with the macrophages without treatment. However, the number of CD206-positive macrophages decreased after coculture with BMMSCs of Rab27a knockdown compared to the BMMSC and BMMSC-ex groups. (e) qRT-PCR analysis of IL-10 and TNF-α in macrophages after being cocultured with BMMSC-, BMMSC/siRab27a-, or BMMSC-derived exosomes (). Macrophages cocultured with BM/siRab27a showed lower IL-10 and higher TNF-α compared to the BMMSC or BMMSC-ex group. Scale bars: 50 μm (b, c). and . Error bars are .

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