α2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro

Figure 6

Identification and analysis of the plasma Evs proteome. Cells cultured in growth medium without induction served as the control group. Lentiviral vectors expressing CFL1, AHSG, APCs, VIM, KIF2A, and TIAM1 were added to the osteogenic differentiation medium in the Ind groups. (a) GO assignment of proteins in plasma Evs according to their assigned fraction and biological function. (b) Biological process. (c) No. of peptides identified. (d) Photographs (top panels) and microscopy images (bottom panels) of ARS and ALP staining in representative samples from the control, induction, and Ind+Lv groups. Original magnification: ×40. (e) The ARS staining in (d) was quantified by monitoring the absorbance at 562 nm. The ALP activity in (d) is presented as units per gram of protein per 15 min. Data were calculated from six to nine samples per group and are presented as .

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