A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells

Figure 2

Venn diagram of proteins detected on LC-MS/MS. There were 1785 types of proteins identified from the mouse primary cultured cell (P0) sample and 1825 types of proteins identified from samples of cells passaged 3 times (P3). Among the 1785 types of proteins in mouse P0 cells, there were 336 types of proteins unique to the primary cultured cells (group P0). A total of 1449 types of proteins in mouse P0 cells were also identified in mouse P3 cells (group P0&P3). Among the 1825 types of proteins in mouse P3 cells, there were 376 types of proteins unique to the cells passaged 3 times (group P3).