A Noninvasive Imaging Toolbox Indicates Limited Therapeutic Potential of Conditionally Activated Macrophages in a Mouse Model of Multiple Organ Dysfunction

Figure 3

Representative images showing a typical cross section of a mouse as obtained by the MSOT both prior to, immediately post-, and 1 minute post-ICG administration (a). Clearance kinetics on day 4 of ICG from a typical healthy mouse (b), an ADR-treated mouse (c), and an ADR+BMDM mouse (d) from a single ischiatic vessel’s region of interest. The blue to red colour scale represents MSOT mean pixel intensity for the resolved ICG signal scaled to the maximum pixel intensity. The change in the mean ICG AUC between days 1 and 4 in all mice is shown in (e) (mean ΔAUC ICG 1.8, ΔAUC ICG 18). Serum ALT was quantified in all mice on day 4 (f). The correlation between the ICG AUC and alanine aminotransferase on day four is shown in (g) (mean 56.1, 218.6, and 266.6 U/l). Each data point represents an individual mouse. .

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