A Noninvasive Imaging Toolbox Indicates Limited Therapeutic Potential of Conditionally Activated Macrophages in a Mouse Model of Multiple Organ Dysfunction

Figure 4

Representative images showing a typical cross section of a mouse as obtained by the MSOT prior to, immediately post-, and 20 minutes post-IRDye administration; cortex and medullary regions of interest are shown (a). The blue to red colour scale represents MSOT mean pixel intensity for the resolved IRDye signal scaled to the maximum pixel intensity. Clearance kinetics on day 4 of IRDye 800 carboxylate from both the kidney cortex and pelvis regions of interest in a typical healthy mouse (b), ADR-treated (c) and ADR+BMDM-treated mouse (d). The change in the mean cortex : pelvis AUC between days 1 and 4 in all mice is shown in (e) (mean ΔAUC C : P -0.085, 0.755, and 2.866). Serum BUN (f) (mean 24.7, 43.4, and 41.1 mg/dl) and SCr (g) levels were quantified on day 4 in each mouse. The correlation between the cortex : pelvis AUC and blood urea nitrogen on day four is shown in (h). Each data point represents an individual mouse. , .

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