Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor

Figure 6

Culture with StemRegenin 1 (SR1) restores stemness of damaged placenta-derived mesenchymal stem cells (PD-MSCs). (a) RT-PCR analysis shows that 1 μM SR1 improves the expression of Oct-4, Nanog, and Sox-2 in 1 μM BP-treated PD-MSCs. One representative of 3 independent experiments is shown. (b) Stemness was evaluated by a CFU-F assay. PD-MSCs treated with 1 μM BP and 1 μM BP with 1 μM SR1 were cultured for 14 days at low density. The data are expressed as the mean ± SD of three independent experiments. . (c) RT-PCR analysis for osteogenesis, chondrogenesis, and adipogenesis was performed in the control, 1 μM BP-treated, and 1 μM BP with 1 μM SR1-treated groups. One representative of 3 independent experiments is shown. (d) The differentiation capacity of PD-MSCs was examined for osteogenesis and chondrogenesis (scale bar, 100 μm; magnification, 200x) and for adipogenesis (scale bar, 50 μm; magnification, 400x) after treatment of 1 μM BP and 1 μM BP with 1 μM SR1 for 14 days. One representative of 3 independent experiments is shown. (e) The concentration of growth factors released from PD-MSCs was measured after treatment of 1 μM BP and 1 μM BP with 1 μM SR1 for 7 days. The data are expressed as the mean ± SD of three independent experiments. In RT-PCR, (−) indicates a negative control using water alone. and .

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