Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

Figure 2

Patch clamp analysis of cardiomyocytes from control- and LQT-iPSC lines. (a) Representative APs of 1 Hz paced a control-iPSC-CM and an LQT-iPSC-CM from II-2 in Figure 1(a). Ten consecutive waves are shown. (b) MDP, APA, and APD₉₀ from cardiomyocytes derived from the six lines: 201B7 (), 409B2 (), 692D2 (), LQT1A1 (), LQT1B1 (), and LQT1C1 (). Data are represented as means ± SEM; . (c) Representative current traces from control- and LQT1-iPSC-CMs. Upper, the protocol in current clamp recording. Middle, representative traces before and after perfusion with 3R4S-chromanol 293B (30 μmol/l). Lower, 3R4S-chromanol 293B-subtraction. (d) I-V plots of I_Ks at the end of the depolarizing step. 692D2 (control) (), LQT1B1 (); .

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