Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

Figure 4

AP measurements in a cardiomyocyte monolayer. Data are represented as means ± SEM. (a) An example phase contrast image of the monolayer culture. Scale bar, 500 μm. (b) Representative AP of a paced control-iPSC-CM monolayer. APD₉₀ was calculated from the average of 10 consecutive waves. (c) APD₉₀ from 3 control- and 3 LQT-iPSC-CM monolayers: 201B7 (), 409B2 (), 692D2 (), LQT1A1 (), LQT1B1 (), and LQT1C1 (). LQT-iPSC-CMs showed longer APD than controls; .

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