Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

Figure 5

AP measurements in cardiomyocyte monolayers using a high-throughput plate reader. (a) Representative AP waves on a 96-well plate. Pacing at 1 Hz starts 10 s after recording. (b) Cisapride prolonged APD₉₀ of both control- and LQT1-iPSC-CMs; 692D2 (control) (), LQT1B1 (). (c) Cisapride prolonged APD₉₀ of both control- and LQT1-iPSC-CMs with 100 nM isoproterenol; 692D2 (), LQT1B1 (). (d) Erythromycin prolonged APD₉₀ of both control- and LQT1-iPSC-CMs with 100 nM isoproterenol; 692D2 (), LQT1B1 (). LQT-iPSC-CM monolayers in 300 nM and 1000 nM cisapride were not synchronized at 1 Hz pacing. Data are represented as means ± SEM; , .

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