Research Article

Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells

Figure 3

Alizarin red staining showed that E2 promoted the production of calcium nodules in a dose-dependent manner. (a) Calcium nodules observed in a microscope. (b) Whole-well images of calcium nodules. (c) Relative quantification of calcium nodules performed in the whole-well images. CM: complete medium; OM: osteogenic medium. Three independent experiments were performed. (. OM group vs. 1-100 nM E2 groups, ).
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