A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells

<div>Differentiation resumes in mature myospheres following inhibition of the notch pathway. (a) Confocal image of whole-mount myosin immunofluorescence on differentiating (9 days) and mature (25 days) myospheres treated for 5 days with DAPT and respective DMSO controls. The effect of notch pathway inhibition is not particularly evident in differentiating myospheres because syncytia continue to leave the myospheres as they are formed. By contrast, DAPT resumes differentiation in mature myospheres, which retain the syncytia. Scale bar: 50 <i>μ</i>m (b) RT-PCR showing the change of hes1 and heyL expression in mature myospheres in response to 5 <i>μ</i>M DAPT treatment. 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Stem Cells International

fig7

Figure 7

Figure 7: A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells 