Research Article
Palmitic Acid Methyl Ester Induces G2/M Arrest in Human Bone Marrow-Derived Mesenchymal Stem Cells via the p53/p21 Pathway
Figure 2
Effects of PAME on cell growth and death in hBM-MSCs. (a) Treatment with PAME (50 and 100 μM) for 48 h inhibited hBM-MSC proliferation. This inhibition was blocked by 0.5% BSA (). (b) PAME (50 μM)-induced proliferation inhibition in hBM-MSCs was confirmed by BrdU assay (). (c) Histogram plots of viable (M1), apoptotic (M2), and necrotic cells (M3) distinguished by flow cytometric analysis using Annexin V-FITC and SYTOX green dye staining. The (d) viable cells, (e) apoptotic cells, and (f) necrotic cells were not significantly different between the PAME-treated group and the control group (). AMA served as a positive control for cell death induction (). All data represent . , versus the control group; #, versus the PAME group. Con: control; AMA: antimycin A; BSA: bovine serum albumin; Veh: vehicle.
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