Palmitic Acid Methyl Ester Induces G₂/M Arrest in Human Bone Marrow-Derived Mesenchymal Stem Cells via the p53/p21 Pathway

Figure 6

Effects of PAME on p53 protein stability. PAME significantly increased the (a) mRNA level of p21 but not p53 () and reduced the (b) level of Mdm2 protein (). The mRNA levels were detected by qRT-PCR, and the protein level was determined by Western blot analysis. (c) PAME prolonged the degradation of p53 protein in hBM-MSCs. The dotted line indicates 50% abundance of p53, and the half-life (t1/2) of p53 protein in each group is shown in the bottom panel. hBM-MSCs were treated with PAME (50 μM) for 6 h followed by CHX (5 μM) treatment and then subjected to protein level detection by Western blot analysis (-6). All data represent . , versus the control group. Con: control; CHX: cycloheximide.

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