Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA

Figure 5

In vitro differentiation capacity of mRNA- or srRNA-iPSCs into the three germ layers: mesoderm, endoderm, and ectoderm. Microscopic images were taken 4 to 5 days after the differentiation of iPSCs, and cells with different morphologies were obtained depending on specific lineage differentiation. After 7 days of differentiation, flow cytometry analyses were performed with two specific antibodies for each lineage (mesoderm: CD31 and α-SMA; endoderm: AFP and CXCR4; ectoderm: Pax6 and Tuj1) and compared to the untreated control. Scale bars represent 100 μm. The results are shown as (). Statistical differences were determined using a paired -test (, , , and ).

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