Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA

Figure 7

Analysis of genomic abnormalities, expression of prooncogenic factors, and the presence of srRNA residues in srRNA-iPSCs. (a) Detection of Klf4 and cMyc oncogene expression in iPSCs generated by srRNA and mRNA delivery using qRT-PCR. Results are shown as (). (b) Representative karyograms of iPSCs generated using srRNA or mRNA. (c) Detection of residual srRNA in obtained srRNA-iPSCs by performing qRT-PCR using nsP2- and nsP4-specific primers. Results are shown as (). (d) Analysis of specific PCR product lengths using 1% agarose gel electrophoresis. nsP2: 192 bases; nsP4: 238 bases; GAPDH: 126 bases. Statistical differences were determined using one-way ANOVA followed by Bonferroni’s multiple comparison test (, , and ).