Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA

Table 1

List of primer sequences used for qRT-PCR analysis.

Gene	Forward primer (5 → 3)	Reverse primer (5 → 3)
Pluripotency marker
GAPDH	TCAACAGCGACACCCACTCC	TGAGGTCCACCACCCTGTTG
Oct4 [3]	AGCGAACCAGTATCGAGAAC	TTACAGAACCACACTCGGAC
Sox2 [3]	AGCTACAGCATGATGCAGGA	GGTCATGGAGTTGTACTGCA
Nanog [3]	TGAACCTCAGCTACAAACAG	TGGTGGTAGGAAGAGTAAAG
Lin28	CTTCTTCTCCGAACCAACC	CAGCCACCTGCAAACTG
E-cadherin	TATACCCTGGTGGTTCAAGC	CACCTGACCCTTGTACGTG
Klf4 [3]	TCTCAAGGCACACCTGCGAA	TAGTGCCTGGTCAGTTCATC
cMyc [3]	ACTCTGAGGAGGAACAAGAA	TGGAGACGTGGCACCTCTT
srRNA-specific marker
nsP2	TCCACAAAAGCATCTCTCGCCG	TTTGCAACTGCTTCACCCACCC
nsP4	TTTTCAAGCCCCAAGGTCGCAG	TGTTCTGGATCGCTGAAGGCAC

GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Oct4: octamer binding transcription factor 4; Sox2: sex-determining region Y-box 2; E-cadherin: epithelial cadherin; Klf4: Krüppel-like factor 4; cMyc: cellular myelocytomatosis; nsP: nonstructural protein.