Histone Arginine Methylation-Mediated Epigenetic Regulation of Discoidin Domain Receptor 2 Controls the Senescence of Human Bone Marrow Mesenchymal Stem Cells

Figure 2

DDR2 was differentially expressed in young adult-derived hBM-MSCs compared to hESCs and hBM-MSCs. (a) Microarray gene expression profiling of young adult-derived hBM-MSCs (Y-hBM-MSCs) and subsequent comparisons with hBM-MSC and hESC GEO data revealed a higher correlation between the gene expression profiles of Y-hBM-MSCs and hBM-MSCs than between Y-hBM-MSCs and hESCs. (b) Bioinformatics analysis identified genes that were either sequentially downregulated or upregulated from hESCs, Y-hBM-MSCs, and hBM-MSCs with at least a 3-fold difference between the adjacent groups. (c) qPCR analysis of in vitro cultured hBM-MSC gene expression further verified the downregulation of two genes, DDR2 and PPRX1, with the increasing cell passage number. P: passage. Bars represent the standard error of the from three repeats. Statistical significance was determined by ANOVA and Student’s -test. , .

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