Comparison of Rapidly Proliferating, Multipotent Aortic Valve-Derived Stromal Cells and Valve Interstitial Cells in the Human Aortic Valve

<div>Comparative analysis of immunophenotype and differentiation abilities on VDSCs and VICs. (a) Immunofluorescent staining for <i>α</i>-SMA, vimentin, SOX-10, and CD146 of VDSCs and VICs. Nuclei are stained with DAPI; scale bars: 50 <i>μ</i>m. (b) Flow cytometric analysis of mesenchymal markers of CD90, CD44, CD29, CD117, CD163, CD133, and CD146 and endothelial markers of CD34, CD31, and CD106 and also hematologic markers of CD11b and CD68 in VDSCs and VICs. (c) Osteogenic, adipogenic, and chondrogenic comparison of the VDSCs and VICs (Alizarin Red S staining for osteogenesis, Oil Red O staining for adipogenesis, hematoxylin staining for the nuclei, and Alcian blue for chondrogenesis); scale bars: 100 <i>μ</i>m.</div>

Stem Cells International

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Figure 3

Figure 3: Comparison of Rapidly Proliferating, Multipotent Aortic Valve-Derived Stromal Cells and Valve Interstitial Cells in the Human Aortic Valve 