Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists

Figure 4

Assessment of the viability and morphology of unstimulated and preconditioned rBM-MSCs. (a) Representative photomicrographs showing the incorporation of calcein AM and its conversion into calcein (green) in live cells. Ethidium homodimer-1 (EthD-1; red) was used to label dying cells. Cells treated with 0.1% saponin were used as a positive control. Scale bar: 200 μm. On the right, quantification of the percentage of live cells in each condition. Preconditioning with polyinosinic-polycytidylic acid (MSC-Poly(I:C)) or lipopolysaccharide (MSC-LPS) did not change cell viability in comparison to unstimulated rat bone marrow-derived mesenchymal stromal cells (MSC; biological replicates). Bars represent the . (b) Representative photomicrographs showing the morphology of phalloidin-stained rat bone marrow mesenchymal stromal cells (red) from the different experimental groups. Nuclear staining with 4,6-diamidino-2-phenylindole (DAPI) in blue. Scale bar: 100 μm.

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