In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds

Figure 5

Representative images of primary limbal epithelial cell cultures at day 4 (a) and day 14 (b) on various carrier materials. At day 4, it is evident that surface modification of CLP hydrogels influences limbal epithelial cell outgrowth, as cell first grew on fibronectin stripes or in grooves, before spreading over the rest of the hydrogel growth. By day 14, cell confluence was reached in a 15 mm diameter outgrowth on both types of RHC I gel, all types of CLP DMTMM gel, and HAM. After 14 days, cells on CLP-12 EDC had not reached full confluence.

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