In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds

Table 2

Tested carrier material.

Abbreviation	Material	Crosslinker	Surface patterning	Collagen concentration
HAM	Denuded HAM	—	—	—
RHC I	RHC I	EDC/NHS	—	~3 mg/cm^2
RHC I F-μCP	RHC I	EDC/NHS	Fibronectin microcontact printing	~3 mg/cm^2
CLP-12 EDC	CLP-PEG	EDC/NHS	—	12%
CLP-12	CLP-PEG	DMTMM	—	12%
CLP-12F-μCP	CLP-PEG	DMTMM	Fibronectin microcontact printing	12%
CLP-12 3D	CLP-PEG	DMTMM	3D topography	12%
CLP-18	CLP-PEG	DMTMM	—	18%

Concentration of RHC I is expressed as net weight at collagen casting (mg/cm²). Concentration of CLP is expressed as percentage of stock solute (%). —: not applicable; HAM: human amniotic membrane; RHC I: recombinant human collagen type I; CLP-PEG: PEGylated collagen-like peptide; EDC: 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide; NHS: N-hydroxysuccinimide; DMTMM: 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride.