Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis

Figure 3

Growth of single sorted Oct4-positive and Oct4-negative cells. (a–c) Representative growth of Oct4-positive and Oct4-negative cells. (a) Growth images of Oct4-positive and Oct4-negative cells. Cells were indicated by white dotted lines. Oct4-positive cells proliferated well compared with Oct4-negative cells. (b) Growth curves of Oct4-positive and Oct4-negative cells. The colony sizes of Oct4-positive and Oct4-negative cells were measured. Oct4-positive cells proliferated well compared with Oct4-negative cells (, ). (c) Survival rates of single sorted Oct4-positive and Oct4-negative cells. The survival rates of Oct4-positive cells were high compared with those of Oct4-negative cells four and eight weeks later of the sorting (, ). (d) Representative image of colony from a single sorted Oct4-positive cell. Oct4-negative cells were produced from Oct4-positive cells. Scale bar, 500 μm.

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