Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro

Figure 5

Proliferation and apoptosis of renal tissues and the tracking of USCs. (a) Proliferation was mainly observed in renal tubular epithelial cells and was increased after USC treatment. Scale bar: 20 μm. (b) Apoptosis was observed in renal tissues and was decreased after USC therapy. Scale bar: 50 μm. (c) Statistical analysis of Ki67-positive cells. (d) Statistical analysis of TUNEL-positive cells. (e) USCs from the P3 generation were transfected with a lentiviral-GFP vector, and the GFP protein was stably expressed in over 90% of USCs. After the tail vein injection of the GFP-labeled USCs, GFP-positive USCs were observed in the frozen sections of the kidney tissues. (A) USCs under bright field. (B) GFP-labeled USCs under a fluorescence microscope. Scale bar: 200 μm. (C) A small number of GFP-labeled USCs were detected at d2. (D) Only a few GFP-labeled USCs were observed at d4. Scale bar: 50 μm. GFP: green fluorescent protein; , .

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