Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability

Figure 1

Generation of 20 reprogrammed cell lines and their classification based on morphology. (a) A total of 20 lines were SSEA4⁺ enriched and passaged >P10 and subsequently microscopically classified into one of the tree morphology groups; stable colony (14 cell lines), unstable class 1 (4 cell lines), and unstable class 2 (2 cell lines). (b) In the upper panel, phase-contrast images illustrate a representative morphology for the different groups and in the lower panel immunofluorescence images showing the organization of the structural proteins α-tubulin and β-catenin. (c) An overview of the 20 reprogrammed lines, organized by donors. The generated lines from each donor are named with the letters A–D. The classification is indicated by colour where black = stable colony, grey = unstable class 1, and white = unstable class 2.

(a)

(b)

(c)