Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability

Figure 4

Variations in pluripotency markers and the ability to separate the stable colony morphology group from the unstable colony morphology group. (a) A heat map of 33 commonly used pluripotency markers [26, 27] identified in our proteomic data set. The cluster analysis was only applied on rows, not columns. The heat map revealed a group of 10 markers that were able to separate the two morphology groups. (b) ROC curves for POU5F1, SOX2, PODXL, and CD9 when comparing the stable colony morphology group with the unstable colony morphology group. (c) Flow cytometry analysis of the markers POU5F1 and CD9 in selected stable (S) and unstable (U) lines.

(a) Pluripotency markers ()

(b)

(c)