Mesenchymal Stem Cell Therapy Facilitates Donor Lung Preservation by Reducing Oxidative Damage during Ischemia

Figure 2

Donor lung preservation rat model. (a) Experimental study design. The procedure included cardiac arrest, warm ischemia, cold ischemia, and normothermic ex vivo lung perfusion using Steen solution with mechanical ventilation. Ablation of the lung was performed after 1 h of warm ischemia, and cell therapy was administered via the pulmonary artery. The lung was mechanically ventilated during perfusion starting with a short alveolar recruitment strategy (#2). Lung functional data were acquired right after the cardiac arrest (baseline, #1 green bar) and at the end of the perfusion (endpoint, #3 green bar). For further details, please refer to Materials and Methods (Section 2.2). (b) Schematic diagram of the normothermic ex vivo lung perfusion step. After the cold ischemia period, the lungs were rewarmed and perfused through the pulmonary artery (PA) with Steen solution for 1 h using an open perfusion circuit in which the left atrium (LA) was opened. During perfusion, the lungs were mechanically ventilated. (c) Representative image of a rat lung during perfusion. Please note the position of the PA cannula (black arrow) for the perfusion and the tracheotube (white arrow) for the mechanical ventilation.

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