Mesenchymal Stem Cell Therapy Facilitates Donor Lung Preservation by Reducing Oxidative Damage during Ischemia
Lung injury and inflammation during organ preservation. (a) Hematoxylin and eosin stained sections obtained at the end of the perfusion from the lungs receiving infusion of vehicle (B) or HUCPVCs (C). Lung sections from untouched rats (basal, (A)) were used as controls. Alveolar septal thickening and interstitial cellular infiltration were more evident in the lungs from the vehicle group. Results show representative images from each group at ×200 original magnification (inset: ×400 original magnification). (b) Representative images of the lung tissue sections with immunostaining against myeloperoxidase (MPO) at ×400 original magnification. MPO-positive cells appear brown. (c) Histological injury scores of the lungs in different groups were quantified as described in Materials and Methods. Data are expressed as . against the basal group derived from one-way ANOVA after multiple comparisons Tukey post hoc test. (d) Lung wet/dry weight ratios at the end of the perfusion. Data are expressed as . (e) Quantitation of MPO-positive cells (from (b)). MPO-positive cells were counted in 10 visual fields/section at ×200 magnification, and the average number for each sample was calculated. Data are expressed as . and # against the basal group derived from one-way ANOVA after multiple comparisons Tukey post hoc test. (f) Lung myeloperoxidase (MPO) activity. Data are expressed as . against the basal group derived from one-way ANOVA after multiple comparisons Tukey post hoc test.
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