Detection of Human Microchimerism following Allogeneic Cell Transplantation Using Droplet Digital PCR

Table 5

Specificity.

Gene	Positive droplets	Control (-)	NTC	Control (+)
SRY	Average number of SRY+ droplets	0	0	53295
	Average number of RNAse P+ droplets	0.875	0.5	49548
RHD	Average number of RHD+ droplets	0	0	10140
	Average number of RNAse P+ droplets	0	0.0833	44913
TRY6	Average number of TRY6+ droplets	0.0417	0	7017
	Average number of RNAse P+ droplets	0.25	0.125	39515
LEC3C	Average number of LEC3C+ droplets	0.0417	0.0417	1325.666667
	Average number of RNAse P+ droplets	0.5833	0.3333	6477
GSTM1	Average number of GSTM1+ droplets	0.0417	0.0417	11861.66667
	Average number of RNAse P+ droplets	20.75	0.4167	11417.75
GSTT1	Average number of GSTT1+ droplets	0.0833	0	12025.5
	Average number of RNAse P+ droplets	0.9583	0.0417	12123.75

NTC: no template control.