Research Article
Detection of Human Microchimerism following Allogeneic Cell Transplantation Using Droplet Digital PCR
Table 7
Percentage of microchimerism detected in patients’ biopsies. LoB: limit of blank.
(a) Patient 1 |
| | Samples | GSTM1 | GSTT1 | | Ratio | >LoB? | % chimerism | Ratio | >LoB? | % chimerism |
| | Preinfusion biopsy | 0 | No | NA | 0 | No | NA | | 3M postinfusion biopsy | 0.0001654 | Yes | 0.03308 | 0.0002596 | Yes | 0.02596 | | 7.5M postinfusion biopsy | 0 | No | NA | 0 | No | NA |
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(b) Patient 2 |
| | Samples | TRY6 | | Ratio | >LoB? | % chimerism |
| | Preinfusion biopsy | 0 | No | NA | | 3M postinfusion biopsy | 0.00000857 | No | NA | | 5M postinfusion biopsy 4 | 0.0000545 | No | NA | | 5M postinfusion biopsy 5 | 0.0000175 | No | NA | | 5M postinfusion biopsy 7 | 0.0000144 | No | NA | | 5M postinfusion biopsy 9 | 0.0000339 | No | NA | | 5M postinfusion biopsy 13 | 0.0000074 | No | NA | | 5M postinfusion biopsy 16 | 0.00000649 | No | NA | | 5M postinfusion biopsy 18 | 0.0001532 | Yes | 0.01532 | | 5M postinfusion biopsy 19 | 0.0000149 | No | NA |
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(c) Patient 3 |
| | Samples | SRY | RHD | GSTM1 | | Ratio | >LoB? | % chimerism | Ratio | >LoB? | % chimerism | Ratio | >LoB? | % chimerism |
| | Preinfusion biopsy | 0 | No | NA | 0.0000237 | Yes | NA | 0 | No | NA | | 10M postinfusion biopsy 1 | 0.0000429 | Yes | 0.00858 | 0.0000705 | Yes | 0.00705 | 0.0000317 | No | NA | | 10M postinfusion biopsy 2 | 0.0000182 | No | NA | 0.0000139 | No | NA | 0.0000194 | No | NA | | 10M postinfusion biopsy 3 | 0.0000093 | No | NA | 0.0000231 | Yes | 0.00231 | 0.0000051 | No | NA | | 10M postinfusion biopsy 4 | 0.0000199 | No | NA | 0 | No | NA | 0 | No | NA | | 10M postinfusion biopsy 5 | 0.0000126 | No | NA | 0.0000127 | No | NA | 0.0000085 | No | NA | | 10M postinfusion biopsy 6 | 0.0000056 | No | NA | 0.0000102 | No | NA | 0.0000096 | No | NA | | 10M postinfusion biopsy 7 | 0.0000252 | No | NA | 0.0000128 | No | NA | 0.0000044 | No | NA |
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