Oxygen Regulates Human Pluripotent Stem Cell Metabolic Flux

Figure 2

Intracellular glucose-derived metabolites accumulate more rapidly under physiological oxygen. (a) ¹³C-glucose fluxomics workflow using NMR. (b–c) Representative 2D ¹H-¹³C HSQC NMR spectra (b). Magnification of NMR spectrum showing representative metabolites (c). Each peak is a quantifiable carbon-hydrogen bond from a metabolite. (d) ¹³C-flux analysis over 0–4 hours of MEL2 hPSC cultured under 5% or 20% oxygen. Measurements are of intracellular metabolite levels. All assays performed in biological triplicate. Error bars represent the SEM. , , , and for paired Student’s -test.

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