Oxygen Regulates Human Pluripotent Stem Cell Metabolic Flux

Figure 4

Global acetylation is increased, and methylation is decreased at 5% oxygen. (a) H3K9ac, H3K27ac, and K3K27me3 fold change of MEL2 hPSC at 5% relative to 20% oxygen. Scale is 50 μm. (b) Workflow for RNA-seq analysis using https://usegalaxy.org/, DAVID, and NetworkAnalayst. (c) Venn diagrams showing the overlap of genes upregulated in two hPSC lines at 5% oxygen and 20% oxygen and total DEG count. (d) Functional clusters with enrichment scores greater than 2 derived from Biological Process and Molecular Function GO terms. (e) Volcano plot of MEL2 hPSC transcriptional response to 5% and 20% oxygen. Red genes indicate a fold change value greater than 2 and an adjusted value (Benjamini FDR) less than 0.05. All assays performed in biological triplicate. Error bars represent the SEM. for paired Student’s -test.

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