Figure 1: Schematic flow chart and timeline of study design. ARDS was induced by administration of Escherichia coli lipopolysaccharide intratracheally (ARDSp) or intraperitoneally (ARDSexp). Control mice (C) received saline solution intratracheally (Cp) or intraperitoneally (Cexp). After 24 h, ARDSp and ARDSexp animals were further randomized to receive saline (50 μL, SAL), bone marrow-derived MSCs (105, 50 μL), or EVs (105, 50 μL), stimulated (MSC serum, EV serum) or not with serum obtained from ARDSp or ARDSexp animals. All data were analyzed on day 2.