Research Article

Scalable Culture Strategies for the Expansion of Patient-Derived Cancer Stem Cell Lines

Table 1

Effect of the microcarrier type on CRC (colorectal cancer) and NSCLC (non-small-cell lung cancer) cell growth using serum-containing medium.

Microcarrier typeCytodex1™PPlus 102-LFact 102-LCytodex3™CGEN 102-LPro-F 102-LCytopore2™CultiSpher®-S

CSC lineCRC
(×104 cell/cm2)2.0
(×104 cell/cm2)
Expansion ratio
CSC lineNSCLC
(×104 cell/cm2)2.0
(×104 cell/cm2)
Expansion ratio

Fold increase in total cell concentration attained at day 6 of culture determined by the ratio between cell concentration achieved at day 6 () and cell concentration used at inoculum (), respectively.