Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells

Figure 8

In vivo migration of untreated or CHIR99021-treated XtiSCs transplanted into the dorsal veins of 3-week-old tadpoles towards skin injury. (a) Differential migration behavior of untreated and treated RFP-expressing XtiSCs inside wounded or unwounded tadpoles. Arrows show the migrating RFP-positive XtiSCs; thick arrows indicate the injection sites. The injury site is marked by a black asterisk. Scale bar: 500 μm. (b) The distance of leading cells from the injection site was measured 6 hours after transplantation by ImageJ and expressed in the chart. (c) Two days after transplantation, fibronectin was detected in untreated XtiSCs at the injury site only but not in cells outside wounded areas in both groups. Results are representative of three biological replicates. Nuclei were stained with DAPI. Scale bar: 20 μm; , .

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