Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats

Figure 1

Phenotypic characterization of rat MSCs. (a) MSC immunophenotypic profiles were detected by using a flow cytometer. The red lines indicate the fluorescence intensity of cells stained with the corresponding antibodies, and the green lines represent isotype-matched negative control cells. (b) Passage 3 MSCs (A) were treated with the osteogenic differentiation medium for 21 days and then stained with alizarin red (B), alcian blue of the MSC pellet after chondrogenic induction for 21 days (C), and oil red O of MSCs after adipogenic induction for 12 days (D).

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