Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices

Table 1

Summary of experimental groups for peripheral innervation cocultures.

	Whole explant control	HC-only control	AN/HC control	SC/HC coculture
Preparation	Whole cochlear explant where HCs and ANs are kept intact	HC-only explant where ANs are dissected from HCs	HC rows and ANs are separated and cocultured	HCs and SC-derived neurons: hair cell rows are cocultured with SC-derived neural progenitors
Culture period	1 DIV	12-14 DIV	12-14 DIV	12-14 DIV
Images
Controls for	Positive control for synapses between HCs and endogenous ANs	Negative control for synapses	For comparison between SC-derived neurons and endogenous ANs in reinnervation	Experimental group

HC: hair cell; AN: auditory neuron; SC: stem cell; DIV: days in vitro.