Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices

Table 2

Summary of experimental groups for central innervation cocultures.

	CN control	SC control	SC/CN coculture
Preparation	Cochlear nucleus slice (200 μm thick)	Human PSC-derived neurons cultured on their own	Human PSC-derived neurons cultured with cochlear nucleus slice
Culture period	1 or 14 DIV	14 DIV	14 DIV
Image
Controls for	The presence of VGLUT1 in cultured CN slice without addition of hPSC	The presence of VGLUT1 in cultured hPSC without CN	The specificity of hPSC-derived neural innervation

CN: cochlear nucleus; SC: stem cell; DIV: days in vitro.