Notch Inhibition Promotes Differentiation of Liver Progenitor Cells into Hepatocytes via sox9b Repression in Zebrafish

<div>N3ICD overexpression impairs Hnf4a induction in BECs. (a) Experimental scheme illustrating the period of MTZ treatment and the stages of heat-shock (red arrows) and analysis (black arrow). (b) Single-optical section images showing Hnf4a and Tp1:H2B-mCherry expression in regenerating livers at A33h. Arrows point to Hnf4a+ BEC-derived cells with round nuclei; arrowheads point to Hnf4a- BEC-derived cells with elongated nuclei. (c) Quantification of the percentage of Hnf4a+ cells among BEC-derived cells, as shown in B. Scale bar: 100 μm; error bars: ±SEM.</div>

Stem Cells International

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Figure 2: Notch Inhibition Promotes Differentiation of Liver Progenitor Cells into Hepatocytes via sox9b Repression in Zebrafish