(a)
(b)
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Figure 5: Osteogenic capability of spheroids. Monolayer-cultured CBDCs and spheroid-derived cells were incubated with osteogenic induction medium for 7 days. ALP assay data showed that induced spheroid-derived cells have significantly increased ALP activity compared with induced monolayer cells (a). qRT-PCR data showed that induced spheroid-derived cells expressed higher levels of osteogenic-related genes, such as osterix, BSP, and DMP1, with statistical significance (b–d). Data are represented as the . ALP assay, ; qRT-PCR, . , , and .