Figure 7: Immunofluorescence staining of neurogenic-induced spheroid-derived and monolayer-cultured CBDCs. After 2 weeks of neurogenic induction, spheroid-derived cells and monolayer-cultured cells were confirmed by immunofluorescence staining. The expression of Nestin and βIII-tubulin was observed with neural cell-like morphology only in spheroid-derived cells (a). In contrast, monolayer-cultured cells showed no positive staining for both Nestin and βIII-tubulin (b). DAPI (4,6-diamidino-2-phenylindole) was used for nuclear staining. Scale .