Research Article
Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability
Table 1
Immunofluorescence staining antibody reagent list.
| | Antibody | Dilution | Product no. and manufacturer |
| | Primary antibodies | | | | SSEA1 (mouse monoclonal) | 1 : 100 | ab16285, Abcam | | Sox2 (rabbit polyclonal) | 1 : 250 | ab97959, Abcam | | Oct4 (rabbit polyclonal) | 1 : 250 | ab19857, Abcam | | Nanog (rabbit polyclonal) | 1 : 100 | ab80892, Abcam | | βIII-tubulin (mouse monoclonal) | 1 : 250 | ab87087, Abcam | | Nestin (mouse monoclonal) | 1 : 500 | ab6142, Abcam | | Secondary antibodies | | | | IgM Alexa Fluor 488 (goat anti-mouse) | 1 : 200 | ab150121, Abcam | | IgG Alexa Fluor 647 (goat anti-rabbit) | 1 : 500 | ab150079, Abcam | | IgG Alexa Fluor 488 (goat anti-mouse) | 1 : 500 | ab150113, Abcam |
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