Table 1: Immunofluorescence staining antibody reagent list.

AntibodyDilutionProduct no. and manufacturer

Primary antibodies
SSEA1 (mouse monoclonal)1 : 100ab16285, Abcam
Sox2 (rabbit polyclonal)1 : 250ab97959, Abcam
Oct4 (rabbit polyclonal)1 : 250ab19857, Abcam
Nanog (rabbit polyclonal)1 : 100ab80892, Abcam
βIII-tubulin (mouse monoclonal)1 : 250ab87087, Abcam
Nestin (mouse monoclonal)1 : 500ab6142, Abcam
Secondary antibodies
IgM Alexa Fluor 488 (goat anti-mouse)1 : 200ab150121, Abcam
IgG Alexa Fluor 647 (goat anti-rabbit)1 : 500ab150079, Abcam
IgG Alexa Fluor 488 (goat anti-mouse)1 : 500ab150113, Abcam