Research Article
Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability
Table 2
Quantitative reverse transcription-PCR primer set list.
| Primer | Direction | Sequence (5-3) |
| β-Actin | Forward | CATCCGTAAAGACCTCTATGCCAAC | Reverse | ATGGAGCCACCGATCCACA |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Forward | GGTGTGAACCACGAGAAA | Reverse | TGAAGTCGCAGGAGACAA |
| Sox2/sex determining region Y (SRY)-box 2 | Forward | GTTCTAGTGGTACGTTAGGCGCTTC | Reverse | TCGCCCGGAGTCTAGCTCTAAATA |
| Fucosyltransferase 4 (FUT4·SSEA1) | Forward | GCAGGGCCCAAGATTAACTGAC | Reverse | AAGCGCCTGGGCCTAAGAA |
| Octamer-binding transcription factor 4 (Oct4) | Forward | CAGACCACCATCTGTCGCTTC | Reverse | AGACTCCACCTCACACGGTTCTC |
| Nanog | Forward | TGCCAGTGATTTGGAGGTGAA | Reverse | ATTTCACCTGGTGGAGTCACAGAG |
| Hypoxia-inducible factors 2α (HIF-2α) | Forward | CAGTACTCCCACAGGCCTGACTAAC | Reverse | GACTGTCACACCGCTGCCATA |
| CD105 | Forward | CTGCCAATGCTGTGCGTGAA | Reverse | GCTGGAGTCGTAGGCCAAGT |
| CD44 | Forward | CAAGCCACTCTGGGATTGGTC | Reverse | GGCAAGCAATGTCCTACCACAAC |
| CD29 | Forward | CCATGCCAGGGACTGACAGA | Reverse | GAGCTTGATTCCAATGGTCCAGA |
| Stem cell antigen-1 (Sca-1) | Forward | TTGCCTTTATAGCCCCTGCT | Reverse | GTCATGAGCAGCAATCCACA |
| Kruppel-like factor 4 (KLF4) | Forward | AACATGCCCGGACTTACAAA | Reverse | TTCAAGGGAATCCTGGTCTTC |
| Transcription factor Sp7/osterix (OSX) | Forward | AGGCCTTTGCCAGTGCCTA | Reverse | GCCAGATGGAAGCTGTGAAGA |
| Bone sialoprotein (BSP) | Forward | GAGACGGCGATAGTTCC | Reverse | AGTGCCGCTAACTCAA |
| Dentin matrix protein 1 (DMP1) | Forward | AGTGAGTCATCAGAAGAAAGTCAAGC | Reverse | CTATACTGGCCTCTGTCGTAGCC |
| Microtubule-associated protein 2 (MAP2) | Forward | CAGTTTGGCTGAAGGTAGCTGAA | Reverse | CACATCTGTGTGAGTGTGTGTGGA |
| Nestin | Forward | GAGGTGTCAAGGTCCAGGATGTC | Reverse | ACACCGTCTCTAGGGCAGTTACAA |
| Nerve growth factor receptor (NGFR)/P75NTR | Forward | TCTGATGGAGTCGGGCTAATGTC | Reverse | CCACAAATGCCCTGTGGCTA |
| Neuronal differentiation (NeuroD) | Forward | CAAAGCCACGGATCAATCTTC | Reverse | TGTACGCACAGTGGATTCGTTTC |
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