Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture

Figure 5

Comparison of growing human-derived colon organoids in Matrigel and in collagen-based matrix culture system. (a) Growth pattern and morphology of human large intestine organoids cultured in both Matrigel and collagen-based matrix during Passage 7. Bar scale is 50 μm. (b) Quantitative RT-PCR was performed to identify specific markers (Lgr5, Muc5AC, Vil1, ChrgB, Muc2, and Lyz) of human large intestine at Passage 4 in both matrices (left). Chromogranin B (ChrgB) was used for targeting enteroendocrine cells. Gene expression shown as gel electroporation (right). , . (c) The immunocytochemistry staining of Ki67, villin, mucin 2, and lysozyme was examined in Matrigel and Collagen matrix. Scale bar: 100 μm.

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