Research Article
Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture
| | Reagent name | Company | Cat. No. | Mouse colon | Mouse small intestine | Mouse stomach | Human colon | Final concentration |
| | Advanced DMEM/F12 | Gibco | 12634-028 | O | O | O | O | 1 X | | Penicillin/streptomycin | Welgene Inc. | LS202-02 | O | O | O | O | 100 U/ml, μg/ml | | B-27 supplement | Gibco | 17504-044 | O | O | O | O | 1 X | | N-2 supplement | Gibco | 17502-048 | O | O | O | O | 1 X | | HEPES buffer solution | Gibco | 15630-080 | O | O | O | O | 10 mM | | GlutaMAX-I | Gibco | 35050-061 | O | O | O | O | 2 mM | | N-Acetylcysteine | Sigma-Aldrich | A7250 | O | O | O | O | 1.25 mM | | Murine recombinant EGF | PeproTech | AF-315-09 | O | O | O | O | 50 ng/ml | | Murine recombinant Noggin | PeproTech | 250-38 | O | O | O | O | 100 ng/ml | | R-Spondin-1 conditioned medium | Home-made | O | O | O | O | 10% | | Wnt3A conditioned medium | Home-made | O | | O | O | 50% | | FGF10, 38-208aa human | ATGen | ATGP1387 | | | O | | 200 ng/ml | | A83-01 | Tocris Bioscience | 2939 | O | O | O | O | 500 nM | | Nicotinamide | Sigma-Aldrich | N0636 | | | | O | 10 mM | | [Leu15]-Gastrin 1 human | Sigma-Aldrich | G9145 | | | O | O | 10 nM | | Y-27632 | STEMCELL Technologies | 72304 | O | O | O | O | 10 μM | | CHIR99021 | Sigma-Aldrich | SML1046 | O | O | O | O | 2.5 μM | | Thiazovivin | Sigma-Aldrich | SML1045 | | | | O | 2.5 μM | | SB202190 | Sigma-Aldrich | S7067 | | | | O | 10 μM |
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