Research Article

MEK Inhibition Targets Cancer Stem Cells and Impedes Migration of Pancreatic Cancer Cells In Vitro and In Vivo

Figure 3

Effects of MEK inhibition on stemness. Gene expression of the indicated stemness-associated genes in (a) adherent cultures or (b) spheres after treatment with vehicle or PD0325901 (PD). (c) Gene expression and (d) RNAseq analysis of murine primary cancer cell lines treated with DMSO vehicle (white) or trametinib (grey) for 48 h. (e) Sphere formation capacity of cells pretreated with PD0325901 at the indicated concentrations. (f) Quantification of large spheres (>120 μm) and representative micrographs of sphere cultures after 7 days. for all experiments. vs. control.