Therapeutic Effects of Intravenous Injection of Fresh and Frozen Thawed HO-1-Overexpressed Ad-MSCs in Dogs with Acute Spinal Cord Injury

Figure 8

Histopathology of injured spinal cords stained with H&E and luxol fast blue. (a) Injured spinal cord segments with fibrosis, hypotrophy, and hemorrhages. (b) Representative H&E staining of injured spinal cord segments. The fibrotic area is observed as a red & pink color, while the healthy area is observed as purple. (c) Representative staining of myelin with luxol fast blue. Positive staining is depicted by the intensity of the blue color. The images were obtained at low magnification 40x with a scale bar of 1000 μm. (d1) % expression of gross lesion area. (d2) % expression of fibrosis relative to normal. (d3) % expression of the myelinated area relative to normal. Each bar represents the average of 4 samples per group. Represents the significance of the HO-1 MSC with the GFP MSC and FT-HO-1 MSC groups at . Arrows denote fibrotic areas; arrowheads denote vacuoles.

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