Research Article

Preconditioning of Human Dental Pulp Stem Cells with Leukocyte- and Platelet-Rich Fibrin-Derived Factors Does Not Enhance Their Neuroregenerative Effect

Figure 1

Workflow to isolate L-PRF subfractions. The L-PRF clot is isolated by centrifugation from blood samples that are subsequently processed for CM L-PRF and EX L-PRF isolation. L-PRF clots are transferred to 6-well plates in culture medium, and CM L-PRF was collected after 96 h. The L-PRF clot is transferred to the Xpression™ Box and by gravitational compression of the clot, EX L-PRF is collected. Both CM- and EX L-PRF were filtered before use. This figure was partly created using Servier Medical Art licensed under a Creative Common Attribution 3.0 Generic License, available online at http://smart.servier.com/.